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KMID : 0380020140290060426
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 6 p.426 ~ p.431
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Effects and Optimization of Gamma-Amino Butyric Acid (GABA) Production Process using Glutamate Decarboxylase (GAD)
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Kim Eui-Jin
Lee Jung-Heon
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Abstract
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pH controlled batch reactor and bubble column reactors have been developed in this research. They were used to produce high concentration of GABA and to determine optimal pH for GABA production. Glutamate decarboxylase (GAD) was isolated from recombinant E. coli and used for GABA production from monosodium glutamate (MSG). pH control was inevitable because the pH increased with MSG consumption. GAD showed highest activity at acidic conditions at pH 5.5 but the optimal pH for GABA production was pH 6.0. When 1.5 mole of MSG was used as reactant, the 1.05 mole of GABA was produced after 10 hrs batch reaction. Using bubble column reactors, 80 % of MSG was converted to GABA for 6 hrs reaction and 1.2 mole of GABA was produced.
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KEYWORD
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Gamma-amino butyric acid, Glutamate decarboxylase, Bubble column reactor
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